The objective of this research is to generate and validate cell-based probes (reporter cells) to non-destructively monitor cell differentiation progression in 3D printed multi-material segmented scaffolds for cartilage and bone tissue engineering.

The approach to assess differentiation events in real-time is based on the generation of cell-based probes to directly interrogate differentiation events during in vitro chondrogenesis and in vitro and in vivo osteogenesis. Native promoters of well-established cell-differentiation biomarkers are used to create independent reporters incorporating a traceable signal (Luciferase, Fluorescent markers), to monitor in real-time the progression throughout the differentiation program.

In collaboration with the CECT, we will incorporate a new platform in order to generate and validate molecular reporters for differentiation events using 3D-printed scaffolds which are more relevant for tissue-engineered applications.